Re: Asp-N Cleavage

Jose Cesar Rosa (jcrosa@fmrp.usp.br)
Thu, 23 Apr 1998 13:49:56 -0300 (EST)

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In reply to: Adams, Greg: "Asp-N Cleavage"

Hi,
I have experienced Asp-N digestion of a glycoprotein 16kDa and I
used the ratio of substrate/enzyme 1:200 (w/w) in 0.05M sodium phosphate
buffer pH 8.0 for 18h at 37C, occured only two cleavage at Glu and the
peptides were less prevalent than others starting with Asp.
I hope this add something
Jose Cesar Rosa

On Wed, 22 Apr 1998, Adams, Greg wrote:

> Hi,
>
> I have a protein that I digested with Asp-N (2% w/w for 24 hours). I
> suspect that I am also getting some minor cleavage at the N-terminal
> side of Glutamic Acid. Has anyone experienced this anomalous cleavage
> when using Asp-N.
>
> Greg Adams
> MA BioServices
>
>

Jose Cesar Rosa, M Sc
Centro de Quimica de Proteinas
Faculdade de Medicina Ribeirao Preto
Universidade de Sao Paulo
Av. Band
eirantes, 3900
14049-900 Ribeirao Preto, Brazil
phone: 55-16-6331884
fax: 55-16-6332119
cell: 55-16-9948642
email: jcrosa@fmrp.usp.br

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