Extraction of a phosphopeptide

Satya Yadav Ph.D. (YADAVS@cesmtp.ccf.org)
Thu, 23 Apr 1998 15:23:26 -0400

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Dear friends,
We have isolated a 3KD peptide and have phosphorylated this with P32.
We tried to extract the peptide from SDS gels using 60% acetonitrile +
0.1% TFA but could be able to extract only about 10% radioactivity. Is
there any other better extraction procedure available? We plan to try
electroelution as a last choice.
Thanks in advance for your suggestions

Satya Yadav
Lerner Research Institute
The Cleveland Clinic Foundation
Cleveland, Ohio

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