Digest - Q-G cleavage by LysC

RLNiece (RLNiece@AOL.com)
Mon, 27 Apr 1998 11:35:19 EDT

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I am trying to rationalize the excision of the tetrapeptide, GGNK, from the
sequence, -QGPPPQGGNKSQGPPP- during LysC digestion. Apparently only 1 of the
3 Q-G sequences in this region was cleaved with efficiency sufficient to beget
enough peptide to purify. Has anyone else observed Q-G cleavage with this
enzyme?

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