ProtSeq: CNBr in situ

Len Packman (lcp2@mole.bio.cam.ac.uk)
Mon, 27 Apr 1998 16:03:30 +0000

A number of proteins in a current sequencing project (PVDF blots) are
N-blocked and we are routinely treating the 'sequenced' sample on the
membrane with CNBr and then resequencing the sample to observe and
(hopefully) deconvolute the few signals which result. Many times this has
been successful, however I note that Lys does not show up at expected
positions; for non-blocked samples, Lys is detected.

So my question - in epsilon-PTC-lysine labile to CNBr treatment and
converted to something other than lysine? Do others observe this?

Len

*********************************************************************
Dr Len C. Packman
Assistant Director of Research
Protein and Nucleic Acid Chemistry Facility
Department of Biochemistry
University of Cambridge
80 Tennis Court Road
Old Addenbrookes Site
Cambridge, CB2 1GA, UK
Tel: +44 (1223) 333639 (including answerphone)
FAX: +44 (1223) 766002
e-mail: lcp2@mole.bio.cam.ac.uk
Visit my WWW page at http://www.bio.cam.ac.uk/proj/adr/PNAC/pnac.html