Re: Manual synthesis reaction vessel
H. Zebroski (zebroski@u.washington.edu)
Thu, 30 Apr 1998 15:23:43 -0700 (PDT)
Michael,
You could have your glass shop (I'm sure you've got a good one at MIT
physics/chemistry) make one for you. I had some made two years ago as
resin coupling reaction chambers. Get a 50 or 100ml tri port round bottom
flask and a 15ml or 30ml Buchner funnel with the stopcock on the stem.
Trim most of the cylinder from the Buchner above the frit (Medium works
best for Wang resin) and cut off a disc from the bottom of the flask and
anneal the two together.You can grip the vessel with a standard small
clamp at the center neck, use stoppers (pennyheads w/ Keck clamps) or
septas and do reagent additions w/syringes or needle vent and agitate
resin with gas purge via the stem with argon. Control bubble rate with the
stopcock then close and switch tubing to vacuum aspirator trap to catch
uncoupled reagent excess if desired and or trap washes.
Good luck. Email at above or zebroski@darwin.com if you have more ?
Still lurking,
Henry
On Fri, 24 Apr 1998, Michael Burgess wrote:
> Dear ABRFer's-
> I am looking to purchase a manual peptide synthesis reaction vessel. The
> scale should be in the 30-100mL range. I have some information from both
> Peptide International as well as Anaspec. Does anyone have any
> comments/advice for this purchase? Also, I am looking for a variable rate
> shaker that has four or more positions. Thanks in advance.
>
> Michael Burgess
> Whitehead Institute
> Cambridge, MA
> burgess@wi.mit.edu
>
>
>