> Chris wrote:
> > I don't know of a review, but if you are by chance doing
> >nucleotides, tetrabutylammonium ion works quite nicely. See, for example,
>
> It's a good idea to remember when using TBA and similar cationic
> ion-pairing reagents that you will never be able to use the column again
> for samples for MS analysis as they will have whopping big m/z 242 peaks
> (right, Hong?) in blank fractions. Also, RBA is exceptionally corrosive to
> silica-based columns and best used on polymeric supports.
>
> David
>
> David A. Schooley
> Dept. of Biochemistry/330
> Univ. of Nevada
> Reno, NV 89557
> schooley@med.unr.edu
> tel: (702) 784-4136; fax (702) 784-1419
It also takes some effort to get rid of TBA from the LC tubings completely. I
believe Kerry Nugent from Michrom has published a paper on RPLC analysis of
peptides that included the comparison of different ion pairing acids,
detergents and pH etc. Sorry I don't have the reference on hand, he can be
reached at 916-888-6498 or MICHROM1@IX.NETCOM.COM, good luck!
Hong Li
Dept. of Molecular Pharmacology
Albert Einstein College of Medicine