>He has two questions:
>
>1. How does one measure the association constant for two
>proteins? (I have suggested using Biacore but we do not have
>access to this methodology.)
>
>2. What is the lower limit for an association constant
>below which one would not expect to be able to observe
>significant dimerization during gel filtration
>chromatography?
>
Mark,
The power of analytical ultracentrifugation was always its unique ability
to measure such interactions quantitatively. You will need a.) an
analytical ultracentrifuge and b.) a physical biochemist. Item a is easy to
find. Item b may be more difficult.
regards
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Gary M. Hathaway, Director
PPMAL - Protein/Peptide Micro Analytical Laboratory
California Institute of Technology
139-74, Division of Biology
Pasadena, CA 91125
http://www.cco.caltech.edu/~ppmal
email Gary: hathaway@caltech.edu
email facility: ppmal@caltech.edu
phone: lab (818) 395-6388 or office (818) 395-2769
FAX (818) 449-3414
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