Dear Members of the Association of Biomolecular Resource Facilities
Several labs have experimented with microporous membranes from 3M as sample
prep substrates for MALDI-TOF MS. There have been a handful of papers over
the last three years:
Purification of Contaminated Peptides and Proteins on Synthetic Membrane
Surfaces for Matrix-Assisted Laser Desorption/Ionization Mass
Spectroscopy, Worrall, et al, Johns Hopkins University School of
Medicine, Analytical Chemistry, Feb 98.
Sample Preparation Using Synthetic Membranes for the Study of
Biopolymers By Matrix-Assisted Laser Desorption/Ionization Mass
Spectroscopy, Worrall, et al, Johns Hopkins University School of
Medicine, Aug 96.
Hedgehog Patterning Activity: Role of a Lipophilic Modification
Mediated by the Carboxyl-Terminal Autoprocessing Domain, Porter et al,
Howard Hughes Medical Institute, Cell, Jul 96.
Polyethylene Membrane as a Sample Support for Direct Matrix-Assisted
Laser Desorption/Ionization Mass Spectrometric Analysis of High Mass
Proteins, Blackledge and Alexander, Bristol-Myers Squibb, Analytical
Chemistry, Mar 95.
Following the the publication of each of these papers, 3M would receive
several requests for samples of the microporous membranes. Most recently,
Amina Woods from Johns Hopkins gave a lecture at the ABRF meeting in San
Diego, and again several requests have come to us. Dr. Woods had the best
results from a high density polyethylene, Lot 113-2. The material is not
commercially available at this time.
We are considering a one-time offer of an experimental polyethylene
membrane for your MALDI work. This material will have properties very
similar to Lot 113-2:
3M EXPERIMENTAL MALDI MEMBRANE Type X51
microporous high density polyethylene membrane
thickness
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? 50 =
=B5m
surface features ? 2 =B5m wheel side
? 4 =B5m air side
filtration efficiency 0.1 =B5m
sheet size 20 X 25 cm
sheets/pack 10
price/pack $500
If you believe that you would buy this material, were it available on a=
one-time experimental basis, please send me a note indicating the numbe=
r of
packs that you are quite confident you would purchase. Don't send a har=
d PO
at this time. We'll use the level of interest expressed to decide wheth=
er
or not to go ahead with this offer. Please respond within two weeks.
Thank you
John Crea
3M Filtration Products
jjcrea@mmm.com
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