Re: hplc: billirubin detection

Rod Levine (rlevine@nih.gov)
Wed, 13 May 1998 09:11:57 -0400

At 07:06 PM 5/12/1998 EDT, you wrote:
>Dear Group,
>
>Does any of you have a method for detection and quantitation of billirubin in
>serum/plasma and purified serum proteins (e.g. BSA)?
>
Umit,

There are a vast number of such assays in the literature, mainly because
hyperbilirubinemia is the most common medical diagnosis in the newborn.
It's a fairly mature field, so you may find many of the references in
papers from 1960-1980 or so.

I'm not sure how accurate you need to be nor whether you need to separate
certain isomers. I think Tony McDonough in San Francisco published several
papers on HPLC assay of bilirubin, but I don't have the references.

Rod

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