We are using 1 mm and 0.5 mm i.d. columns quite routinely at BMS for protein
and peptide separations on-line with mass spec. In sample limited situations
the smaller i.d. columns always win. It seems that the major problems
encountered (according to most people out there) are due to large gradient
delay volumes and large volume flow cells. We have fixed that problem by
using Michrom BioResources instrumentation for all of our micro-column work.
We have both the older UMA system and the newer MAGIC system. Not to sound
too "salesy", but it has been our experience that both systems work extremely
well for these applications right out of the box (no re-engineering required).
Mark Hail
Mark Sanders
Bristol-Myers Squibb
_______________________________________________________________________________
From: Harry Whatley on Wed, May 13, 1998 4:04 PM
Subject: Discussion:1 mm HPLC columns
To: Recipients of ABRF List
ABRFers using 1 mm columns:
The recent dmessages concerning 1 mm columns has raised some questions
in my mind. I tried using these several years ago without much success.
I would like to start a discussion on this topic. If you are using
these columns (or have tried to use them), and would like to participate
in this dialog, I offer the following questions as starting points.
1)What applications are being done on 1 mm columns?
2)What advantages do you obtain from the 1 mm column (vs. a larger
diameter)?
3)What are the problems encountered? How are they solved?
Please post your answers to the discussion group.
Regards,
Harry
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Date: Wed, 13 May 1998 07:56:44 -0700 (PDT)
From: Harry Whatley <h_whatley@hotmail.com>
Subject: Discussion:1 mm HPLC columns
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