membrane spanned peptides

Ulf Hellman (ulf.hellman@licr.uu.se)
Thu, 14 May 1998 20:30:33 +0200

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Hi all,
I recall a very particular solvent system for RPC of hydrophobic samples:
Solvent A is 60 % formic acid in water
Solvent B is 60 % formic acid in acetonitrile
Run 0-100 % B in an appropriate time.
We were using this with Vydac C4 columns and could indeed elute some very
hydrophobic samples. Of course forget about low UV detection...
If I remember correctly, I got this from Werner Machleidt in the early
eighties.
/Ulf
Ulf Hellman.
Ludwig Institute for Cancer Research
Box 595
S-751 24 Uppsala
Sweden
Phone +46 18 160423 Fax +46 18 160420
e-mail ulf.hellman@licr.uu.se