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On Wed, 13 May 1998, Suming Hua wrote:
> I have a problem regarding separation and purification of membrane spanned
> peptides with RP-HPLC. I labeled calcium ATPase on the membrane domain,
> then removed the cytosolic parts of the enzyme with trypsin digestion, and
> pelleted the membrane parts which was dissolved in formic acid, then
> filtered. The labeled peptide was separated and collected with c-18 column
> using TFA-acetonitrile system. When I did further purification, the
> peptide was stuck in the column and nothing came off at all. I don't know
> if there are any other methods to deal with the membrane peptides using
> HPLC system.
>
> How can I identify the labeled peptide in the mixture with mass
> spetrometry (the molecular weight of this peptide is 7-8K)? Is it possible
> to load the mixture on mass spectrometer directly? Thank you in advance
> for your kind advices.
>
>