We have received a request to synthesize a peptide and to make a peptide/KLH
conjugate
involving a short (15 residue), cyclic (disulfide bridge) peptide with an
extra (3rd)
Cysteine, at the N-terminus, for coupling to KLH. The N-term Cys was just
added to the
sequence to help in coupling to the carrier, as the presence of internal Glu
and a couple of Lys residues (and absence of Tyr) in the sequence would
probably
eliminate alternative coupling schemes (coupling through an internal AA not
an option).
While it was suggested to us that we could pursue a scheme involving a synthesis
of peptide with 2 internal Cys(Acm) and free N-term Cys, coupling of this
peptide
to KLH and deblocking/oxidation of the conjugate, we would like to hear
some opinions
and suggestions from those of you who dealt with such or similar problems.
Jan Lukszo
Dr. Jan Lukszo
Peptide Analysis and Synthesis
NIH/NIAID/SBS
Twinbrook II
12441 Parklawn Drive
Rockville, MD 20852
phone:(301)496-3786
fax:(301)480-2618
E-Mail: lukszo@fcrfv2.ncifcrf.gov