Re: 1st dimension gels

Ulf Hellman (ulf.hellman@licr.uu.se)
Wed, 20 May 1998 10:20:45 +0300

At 14:40 980519 -0400, you wrote:
>
>Would thioglycholate be just as good as mercaptoacetic? I'm new at this:
>what do you mean that the protein is blocked in the 1st dimension gel?

I just recalled an ol' ref in this matter: See Moos et al. JBC 263,
6005-6008, 1988. They prerun their gels with sodum thioglycolate.
/Ulf
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