we have been using cation exchange on a gram scale preparative scale for
our synthetic peptides for more than ten years and would be horrified to
have to spend this sort of money on a column.
Consider buying yourself a good moderate pressure empty column (we like the
Pharmacia XK series) and pack it with either Pharmacia S Sepharose HP or
BioRad MacroPrepS, we are using the latter at the moment because of its
availability in serious bulk quantities. I expect the total cost will be
less than $2000 for a setup big enough to bind many grams of recombinant
protein, if you choose a large enough diameter column (ie short and fat)
you will be able to achieve flow rates in the 20 to 50 ml/minute range. The
great thing about this approach is that, when you have pumped so much
garbage on the column that the top is discoloured and you're a bit
concerned about the chromatographic resolution, just open it up, suck off
the discoloured material and off you go again.
Save your money!
Regards....Ken
>Hi all,
>
>we are considering the purchase of a cation exchange preparative column
>to purify around 300 mg of a recombinant protein per run. We are looking
>for a good compromise between resolution, high flow rate and cost.
>Our options at the moment are :
>Shodex SP-2025 - 20x150mm - 10um - 1000A - $4000
>Perseptive Biosystem PorosSP/H- 10x100mm - 10um - $3400
>Macherey Nagel Nucleogel SCX - 25x150mm - 10um - $4200
>
>We are very attracted by the high flow rate, loading capacity and separation
>speed permitted by the Poros packings but we wonder about its resolving
>power..
>Does anybody of you have some experience with these columns or do you
>have some other good option to recommend?
>
>Thanks in advance for any help!!!
>
>
>Corrado Guarnaccia
>Protein Structure and Function
>ICGEB - Padriciano 99
>34012 Trieste - Italy
>E-Mail: guarna@icgeb.trieste.it
>Tel: +39-40-3757341
>Fax: +39-40-226555
>
>
********************************
Ken I. Mitchelhill
The John Holt Protein Structure Laboratory
St. Vincent's Institute of Medical Research
41 Victoria Parade
Fitzroy 3065 Victoria
AUSTRALIA
Telephone: 61-3-9288 2480
Facsimile: 61-3-9416 2676
Email: k.mitchelhill@medicine.unimelb.edu.au
Laboratory: http://www.medstv.unimelb.edu.au/WWWDOCS/SVIMRdocs/JHPSL.html
ABRF: http://www.abrf.org
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