Re: HPLC preparative IEC columns
POLYLC (POLYLC@AOL.com)
Tue, 26 May 1998 10:17:07 EDT
This is in response to Ken Mitchelhill's advice to Corrado Guarnaccia to use
medium pressure materials for cation-exchange of synthetic peptides. That
combination would be fine if you only wanted to get rid of deprotection
fragments and to perform a preliminary fraction of the product of an
inefficient synthesis prior to reversed-phase HPLC. There will be cases where
reversed-phase HPLC (RPC) won't have the necessary selectivity - e.g., a
failure sequence minus a ser- residue or some such. In such cases, you'll
need all the efficiency you can get at the cation-exchange step, and an
expensive SCX column becomes unavoidable. If it's any consolation, they have
four times the loading capacity of a RPC column of the same dimension.
Andy Alpert
PolyLC Inc.
Columbia, MD USA