Re: Diferent recovery of Arg in sequences

Katheryn Resing (Katheryn.Resing@Colorado.EDU)
Wed, 27 May 1998 08:31:16 +0000

I have also had the experience of low recovery of arginine at one site, but
not another. At the time, someone told me that arginine is limiting in
bacterial expression systems and that you can get other amino acids in that
position, as well as arginine precursors. It was supposed to be from one
set of the arginine codons, but not the other and the problem that they had
was fixed by changing the low recovery codon and enriching the medium for
arginine. I never got references on it and if anyone out there knows them,
I'd like to have them for my files.

Katheryn Resing