Re: papain cartridge

Dan Crimmins (crimmins@pathbox.wustl.edu)
Thu, 28 May 1998 08:33:41 -0500 (CDT)

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At 11:24 PM 5/27/98 EDT, Wujx@AOL.com wrote:
>Hi, ABRF members:
>
>Did anyone ever use immobilized papain cartridge (provided by Perseptive
>Biosystem) to generate antibody Fab and Fc fragments? I am interested in how
>to generate the fragments in the cartridge and how to elute the fragments from
>the cartridge. Thanks in advance.
>
>Jiang
>
>
>

Jiang,
Can't speek to the Perspective product, but Pierce sells kits
containing immobilized papain/pepsin/ficin. All three work like champs in a
simple "shake and bake" format!

Dan L. Crimmins
Washington University School of Medicine
Dept. Pathology/Division of Laboratory Medicine
660 S. Euclid Ave., Box 8118
St. Louis, MO 63110
Phone: 314-454-8514; Fax: 314-454-5208
e-mail: crimmins@labmed.wustl.edu

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Maybe in reply to: Wujx@AOL.com: "papain cartridge"