IPG strips and PhastSystem 2D electrophoresis

Rich Root (theroots@earthlink.net)
Thu, 28 May 1998 10:53:25 -0700

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I am interested in running some 2-D electrophoresis gels on the
Phastsystem and would like to use IPG strips for the first dimension. I
have seen Gorg's paper on this, but have not been able to obtain the
details on pouring my own 3-9 pH gradient strips. Pharmacia tech support
has been of little help in this, only able to provide methods for
pouring 1-3 pH unit gels. Does anyone have a useful procedure for this?
Also, any tips on running 2Ds on the phastsystem or in general would be
appreciated.

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