Low Arg recoveries
Reed Harris (reed@gene.COM)
Thu, 28 May 1998 10:46:26 -0800
> I have also had the experience of low recovery of arginine at one site,
but
> not another. At the time, someone told me that arginine is limiting
in
> bacterial expression systems and that you can get other amino acids in
that
> position, as well as arginine precursors. It was supposed to be from
one
> set of the arginine codons, but not the other and the problem that
they had
> was fixed by changing the low recovery codon and enriching the medium
for
> arginine. I never got references on it and if anyone out there knows
them,
> I'd like to have them for my files.
>
> Katheryn Resing
Per Katheryn Resing's query:
The use of AGA as an Arg codon in E. coli can lead to Lys mistranslation
(1). CGA as an Arg codon has been mistranslated as Gly (2).
1) Seetharam et al., (1988) Biochem. Biophys. Res. Commun. 155; 518-523.
2) Lange et al., Poster M88 at the 1992 Protein Society meeting.
--
Reed Harris
Genentech, Inc.
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