Susana
If I remember from an earlier question, you have a 477A. If you are getting
variations in the dry time after TFA cleavage, then you may get the variable Arg
that you are experiencing. With respect to your earlier question concerning 5
1/4 disks, have you considered acquiring the 610A data collection system - then
you will have fewer storage problems?
Best wishes
Stephen Bayne
_______________________________________________________________________________
Subject: Diferent recovery of Arg in sequences
From: Susana Linskens <linskens@qb.ffyb.uba.ar> at Internet
Date: 26/05/1998 16:18
Dear all;
As always, I am looking for experience out there.
I am routinely control the first 15 AAs of a recombinant protein that has
Arg in position 4 and
position 10. It has a Cys in position 6 that normally is derivatized before,
with 4-vp. This protein has carbohydrates chains also, in several positions;
the closest, from the primary structure point of view, is in positon 36. In
the last runs, I observed that Arg 4 shows a very low recovery, much less
that I would expect and even less than Arg 10. I checked the sequence, on a
sample without derivatization of Cys but the situation is the same.
Does anybody have idea of a methodological reason of that?. Ssomething in
the protein itself that would explain that result?.
Thanks in advance for your suggestions.
Susana
Susana Linskens
LANAIS-PR0
Junin 956.
1113 Buenos Aires. ARGENTINA
linskens@qb.ffyb.uba.ar
FAX: University:54-1-963-9677
Private: 54-1-807-3235
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From: Susana Linskens <linskens@qb.ffyb.uba.ar>
Subject: Diferent recovery of Arg in sequences
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