Protein Sequencing PVDF
James Farmar (jfarmar@nybc.org)
Fri, 29 May 1998 12:01:13 -0400
A researcher here has been submitting samples to me blotted on Dupont/NEN
Polyscreen PVDF (P/N NEF1000). She thinks she has placed plenty of
digested protein (thus having a free amino terminus) in her gels and the
Coomassie stain of a gel looks that way.
When she blots the non-stained gel to the aforementioned pvdf, she gets
what I call a "foveal" band (the kind you can only see when you don't look
directly at the band (an old astronomy trick)). I get only a few
background amino acids (1 pmol or less) when I sequence.
Has anyone used this pvdf with success? Has anyone noticed any problems
with it?
Thank you for your ideas and help.
Dr. Jim Farmar
MicroChemistry
L.F. Kimball Research Institute
New York Blood Center
212 570-3128
212 570-3195