John M. Stewart, Department of Biochemistry
Univ. of Colorado Medical School, Denver, CO 80262
Phone: 303-315-7534; FAX: 303-315-8215
Email: John.Stewart@UCHSC.edu
On 5 Jun 1998, Rebecca Ettling wrote:
> I provide peptides to my customers after lyophilization from 50% Acetonitrile.
> I do not have an HPLC so I do not offer them that option.
> I
> have a customer that complains that whenever he buys a peptide from me and
> dissolves it in water to use in his assay, that the pH is around 2 -- too low
> for his cells.
>
> He does not want to buffer his solution, but rather wants me to do something
> on my end.
>
> Any ideas ?
>
> What do other people do to get around this problem ?
>
> Thanks in advance -- sometimes you-all are the only sanity in my world.
>
> Rebecca P. Ettling
> Biotechnology Resource Laboratory
> Protein Sequencing and Peptide Synthesis Facility
> Medical University of South Carolina
> 173 Ashley Avenue, Room 733D BSB
> Charleston, SC 29403
> Tel.: (843) 792-1271
> Fax: (843) 792-1264
>
>
>