Sebastian gave you one solution for making stable radioactive peptides.
You can also make peptides for tritiation using dehydroproline or
dehydroalanine residues during your synthesis. This gives you somewhat
more flexibility if a tyrosine would be inappropriate in your peptide. Of
course, the radiolabeling procedure is expensive, and they make much more
hot peptide than you can store in your lab. A cheaper solution, if your
peptide's activity permits, is to use high specific activity radiolabeled
acetic anhydride to acetylate the N-terminus. You can do this in your own
lab, and make a smaller amount of material. Other relatively cheap
solutions are to insert a cysteine into your sequence and label with the
iodoacetic acid or iodoacetamide, again, if your peptide's activity permits.
Ruth
At 11:45 AM 6/3/98 -0600, John Phipps wrote:
>Hello.
>Does anyone know of a contact for synthesis of radiolabeled peptides?
>////////////////////////////////////////
>John Phipps
>Manager Macromolecular Resources
>Colorado State University
>jphipps@mmr.bmb.colostate.edu
>WWW=http://mmr.bmb.colostate.edu/
>FAX:1-800-436-2329
>////////////////////////////////////////
>
>
>