Dan
Consider using a small dialysis chamber and dialyzing against 60mM NaHCO3
at pH 9 and tempo+. It seems easier, faster and cheaper than a column.
Call if you need further information.
Regards,
Amos
Amos Heckendorf (nestgrp@world.std.com)
The Nest Group, Inc., Value Added Resellers of HPLC Columns (Vydac=81,
PolyLC=81, Hydrocell=81, BioChrom Labs=81, Jones, Jordi-Gel=81, Nucleosil=
=81) and
Isolute=81 SPE cartridges; ESA=81 Electrophoresis PAGE mini-gels and Sial=
omed=81
COZAP Destaining pads and MicroDialysis tools; and Quantum Magnetics=81
Immunomagnetic Affinity Particles . Tel: 800-347-6378 or 508-481-6223; F=
AX
508-485-5736;
45 Valley Rd, Southboro, MA 01772-1306
HPLC Applications and Protocols at:
HTTP://world.std.com/~nestgrp/protocols/protocol.html