Re: Polyclonal antipeptide antibody problem
Gregory Grant (ggrant@pharmdec.wustl.edu)
Fri, 12 Jun 1998 03:48:30 -0600
>Hi everyone,
>
>I am raising an antipeptide antibody against a peptide (CGSCVDGRCCTP). The
>synthetic peptide is couplied to KLH using glutaraldehyde. The rabbit
>failed to produce any antibody. We think that the Cs may be the reason
>for this failure. Please give me some suggestions so that I can overcome
>this problem. Best Wishes.
>
>Bob Lee
Bob,
The glutaraldehyde is not a good choice for this peptide because it
will react with the Cys also. See Current Protocols in Protein Science
Chapter 18.3 for more info than I can put into an email.
Greg
Gregory A. Grant
ggrant@pharmdec.wustl.edu