P2D: Maximizing peptide extraction from gels?
Rob Becklin (rbecklin@utmem1.utmem.edu)
Mon, 15 Jun 1998 18:09:55 -0500
Does anyone know what the best way is to optimize the extraction of
trypsin-generated peptides from SDS-PAGE gels? There are many methods out
there that use solvents such as formic acid/water/isopropanol or
acetonitrile/ammonium bicarbonate or just formic acid with or without
sonication, etc. Anyone know which one works best? Thanks in advance!
Regards,
Robert Becklin
University of Tennessee, Memphis
956 Court Ave., Room A218
(901) 448-5488
rbecklin@am.utmem.edu