HPLC fluorescence detectors

Deb McMillen (mcmillen@morel.uoregon.edu)
Tue, 16 Jun 1998 14:54:42 -0700 (PDT)

Hi, all,
We are setting up an HPLC system for chromatography of fluorescently
labeled amino acids. We will be using an older HPLC (Beckman, probably,
or an ABI 120) to do the chromatography. We'll be starting with the
Accq-Tag system, but we will be using other fluorescent probes on the
system in the future. We are looking to purchase a fluorescence detector
with independent programming of both the excitation and emission
wavelengths. The only recorders we currently have on hand for this
project are an SP 4270 and an HP 3396.

I'd like advice as to which of the following detectors would serve us well
in a stand-alone mode--and which data collection systems you would suggest
tying in with the detector to have the most versatility in further
handling of the data. Both the SP 4270 and HP 3396 are frustrating in the
lack of our capability to move the data into another computer system for
data archiving, manipulation and publication.

The fluorescence detectors are

Waters 474 scanning fluorescence detector

HP 1046 Programmable fluorecence detector

McPherson FL-750BX/D HPLC variable excitation wavelength fluorescence
detector (emission analyzer is filter selectable)

Hitachi Fluorescence Detector Model L-7480

Varian Star 9075 Fluorescence Detector

Thanks for any input on these systems,
Deb McMillen
Institute of MOlecular Biology
University of Oregon
Eugene OR