It would seem difficult to select a random peptide even if you keep the
same amino acid composition. A better imperfect control is to mix the
resins in the amino acid composition of the first peptide and split them
for the coupling reactions. That way, the amino acid composition of some
peptides may differ from your first peptide, yet the molar ratio of any one
unique peptide is so low that sequence specificity may not matter in the
experiment. That is how I do my antisense controls in the DNA world. For a
peptide, I would contact PepSyn corporation at 1-800-899-3436 because they
are one of the few places that still do good peptide synthesis manually,
and therefore can give you such custom service without an excessive amount
of work.
To answer your question, it is easy to write a random sequence generator
with Excel. You just have to enter a random number generator, e.g.:
RAND()*100, next to each of the positions of the original sequence, then
push F9 to recalculate, and sort both columns by ascending order of the
random number column. I just wrote one in less than 5 minutes and tested
it. It can look like this:
RAND()*100 K
RAND()*100 L
RAND()*100 M
RAND()*100 E
RAND()*100 T
RAND()*100 K
Good luck to you!
Tony
At 01:29 PM 6/19/98 -0500, Mary Bower wrote:
>Dear Colleagues;
>
>I have a customer who will be ordering a specific peptide for synthesis,
>and she also wants a randomized sequence of the first peptide sequence.
>Does anyone know of a program available that would generate a completely
>random seqence for her. Does anyone have any reccomendations??
>
>Thanks for your help!!
>
>Mary
>
>Mary Bower
>Instrument Specialist
>Purdue University
>Department of Biochemistry
>West Lafayette, IN 47907
>
>Phone (765)494-6540
>Fax (765)494-7897
>email; Bower@Biochem.purdue.edu
>
>
>
>
************************************
Dr. Anthony T. Yeung, Ph.D.
Director, Fannie E. Rippel Biotechnology Facility
Member, Institute for Cancer Research
Fox Chase Cancer Center
7701 Burholme Ave. Philadelphia, PA 19111
Voice: 215-728-2488
FAX: 215-728-3647
email: AT_Yeung@FCCC.edu
http://www.fccc.edu/research/labs/yeung/
************************************