Stripping Auto.DNA sequencing plates
Emily Jackson-Machelski (ejm@aecom.yu.edu)
Mon, 22 Jun 1998 12:51:19 -0500
Does anyone have a reliable way to strip contaminants from glass plates
used for automated DNA sequencing - specifically for ABI 373s and 377s? We
have been cleaning the plates daily with a hot water rinse followed by a
wash cycle without detergent in a dishwasher per ABI instruction. We are
still getting some sort of buildup that causes very short reads (250-300
bases) and sometimes causes sequence to stop around 150 bases, start again
and then stop again. ABI tech service has recommended we try the 2M NaOH
strip method but I have heard that it may not be the best way to clean the
plates. I am also not completely convinced that the problem is entirely
caused by dirty plates. If anyone has any suggestions, I would love to
hear them. Thanks!
Emily Machelski
DNA Sequencing Facility
Protein and Nucleic Acid Chemistry Laboratory
Washington University School of Medicine
660 S. Euclid Ave, Box 8103
St. Louis, MO 63110
ejm@pharmdec.wustl.edu
(314) 362-0283