> 2. Western
> No. First, some kind of blocking agent has to be used and usually it
> is a protein (or protein mixture).
Not necessarily. Tween 20 can be used as a blocking agent.
Corresponding protocol for cytochrome P450 isozymes is described in
Meth. Enz., vol. 206. I've used this technique for years and it
worked beautifully.
M. Alterman, Ph.D.
Director,
Biochemical Research Service Laboratory
University of Kansas
Lawrence, KS 66045
Phone: (913)-864-4166
(913)-864-4247 lab
FAX: (913)-864-5396
http://www.idl.ukans.edu/anylresc/brsl/brsl.html