Re: high sensitivity AAA

Ray DeAngelis (deangerd@email.med.yale.edu)
Fri, 26 Jun 1998 08:47:48 -0400

>Subject: Re: high sensitivity AAA
>
>Ray-
>
>For the past year and a half we have been using the Waters AccQ-Tag system
>for AAA (an AQC derivitization). It works quite well. We routinely inject
>samples in the 10 to 25 pmol level. We run ours on a Waters Alliance HPLC
>with a fluorescent detector, and the Millennium software makes the
>analysis and quantition really easy. It is a pre-column method, and your
>right, salts can adversely effect derivitization. But with clean samples I
>have found the method to be very reliable.
>
>Hope this info is of some use.
>
>
>>>Date: Thu, 25 Jun 1998 13:40:31 -0400
>>>From: Ray DeAngelis <deangerd@email.med.yale.edu>
>>>Subject: high sensitivity AAA
>>>X-Sender: deangerd@email.med.yale.edu
>>>Old-To: abrf@aecom.yu.edu
>>>MIME-version: 1.0
>>>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>>>Sender: Association of Biomolecular Resource Facilities
>>><abrf-request@aecom.yu.edu>
>>>Precedence: bulk
>>>
>>>Hello,
>>>
>>>We are considering upgrading our amino acid analysis service and would like
>>>recommendations on available instruments. We are currently running a
>>>Beckman 6300/7300 analyzer but would like to increase sensitivity to the
>>>low picomole level. (We are also concerned about the long term support of
>>>this instrument from Beckman). Although we prefer an ion exchange
>>>separation/post column detection methodology as it affords the greatest
>>>possible latitude in sample loading conditions, we would appreciate
>>>recommendations on any high sensitivity/robust AAA technology in use. We
>>>are not up to date on the available instrumentation so any recommendations
>>>or suggestions would be helpful.
>>>
>>>Thank you in advance.
>>>
>>>******************************************************
>>>Ray DeAngelis
>>>HHMI Biopolymer / Keck Foundation Biotechnology
>>> Resource Laboratory at Yale University
>>>http://info.med.yale.edu/wmkeck
>>>203/737-2636 telephone
>>>203/737-2058 FAX
>>>
>
>
>
>
>