Re: SEC Terminology
Dan Crimmins (crimmins@pathbox.wustl.edu)
Fri, 26 Jun 1998 10:17:42 -0500 (CDT)
At 08:42 AM 6/26/98 -0400, Kenneth Williams wrote:
>Does anyone have a reference that indicates the difference between these
>three terms or may they be used interchangeably?
>
>(GFC) Gel Filtration Chromatography
>(SEC) Size Exclusion Chromatography
>(GPC) Gel Permeation Chromatography
>
>Thanks,
>
>
>Ken Williams, Ph.D.
>Director, HHMI Biopolymer/Keck Laboratory
>Professor (Adjunct) Research
>Molecular Biophysics and Biochemistry
>
>
>Visit the HHMI Biopolymer/Keck Laboratory Web Page at:
>
> http://info.med.yale.edu/wmkeck/
>
>
>
Ken,
See chapter 3 of "HPLC of Biological Macromolecules" written by K.M.
Gooding & F.E. Regnier. Fred is one of the pioneers of modern HPLC and
answers your questions in the first two paragraphs of this chapter. Editors
--- Gooding & Regnier, Marcel Dekker, Inc., New York, 1990.
Regards,
Dan L. Crimmins
Washington University School of Medicine
Dept. Pathology/Division of Laboratory Medicine
660 S. Euclid Ave., Box 8118
St. Louis, MO 63110
Phone: 314-454-8514; Fax: 314-454-5208
e-mail: crimmins@labmed.wustl.edu