We are trying to electrotransfer small (7-10 residues) basic peptides from
Tricine-SDS gels to PVDF. We have tried the usual CAPS / 20% methanol procedure
but the yield is low. Can anybody recommend a better blotting protocol for small
basic peptides.
Best Regards
Jan J. Enghild
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| Jan J. Enghild Ph.D |
| Duke University Medical Center |
| Department of Pathology P.O. Box 3712 |
| Durham, NC, 27710 |
| USA |
| Phone: (919) 684 2872 |
| Fax: (919) 684 2920 |
| e-mail: enghi001@mc.duke.edu |
| |
| |
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