protein modification

Kenneth D. Hapner (khapner@montana.edu)
Wed, 01 Jul 1998 15:51:30 -0600

Dear abrf: We have a protein (40K) that tends to aggregate to trimers,
tetramers and above. We wish to stabilize the dimer or better, the monomer.
How to do this? I remember the succinic anhydride acetylation procedure to
replace positive lysine groups with negative succinyl groups and will
likely try this. Are there other approaches that we might try? Thanks
Ken Hapner
Montana State University
khapner@gemini.oscs.montana.edu

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Kenneth D. Hapner
Department of Chemistry and Biochemistry
Montana State University
Bozeman, MT 59717-0310
khapner@gemini.oscs.montana.edu