Re: Digest

Jelle Lahnstein (jlahnste@waite.adelaide.edu.au)
Fri, 3 Jul 1998 17:44:03 +0930 (CST)

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In reply to: PMotchnik@AOL.com: "Digest"

Paul, the breakdown of urea is pH and temperature dependent, I carry out
LysC digestions in 4M urea + buffer and some DTT (scavenger) at pH 7 and
RT to minimise (avoid?) carbamylation.

On Thu, 2 Jul 1998 PMotchnik@AOL.com wrote:

> Hi,
>
> How common is carbamylation during trypsin digestion in the presence of
urea?
> We use ultrapure urea and prepare solutions fresh but do not treat with
ion
> exchange resin. Thanks in advance.

Jelle

____________________________________________________
Jelle Lahnstein
Nucleic acid and Protein Chemistry Unit
Department of Plant Science
Waite Campus, University of Adelaide
Glen Osmond, 5064, Australia
Tel : 61 - 8 - 83037260
Fax : 61 - 8 - 83037130
Internet : http://www.waite.adelaide.edu.au/NAPCU/

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