Even scrupulously clean urea can break down, especially at the (usually)
elevated temperatures of a tryptic digest, and potentially lead to
carbamylation. Thus, any digests containing urea should also contain a
scavenger to avoid additional modifications.
Mike Klein
Amgen, Inc.
> ----------
> From: PMotchnik@AOL.com[SMTP:PMotchnik@AOL.com]
> Sent: Thursday, July 02, 1998 4:59 PM
> To: Recipients of ABRF List
> Subject: Digest
>
> Hi,
>
> How common is carbamylation during trypsin digestion in the presence
> of urea?
> We use ultrapure urea and prepare solutions fresh but do not treat
> with ion
> exchange resin. Thanks in advance.
>
> -Paul Motchnik
> XOMA Corp.
>