We normally soak the glass plates with NaOH 2M as you mentioned for
2-3 hs or overnight. We're having good results an no problems.
Leo
> Date: Mon, 22 Jun 1998 12:51:19 -0500
> From: ejm@aecom.yu.edu (Emily Jackson-Machelski)
> Subject: Stripping Auto.DNA sequencing plates
> To: Recipients of ABRF List <abrf@aecom.yu.edu>
> Does anyone have a reliable way to strip contaminants from glass plates
> used for automated DNA sequencing - specifically for ABI 373s and 377s? We
> have been cleaning the plates daily with a hot water rinse followed by a
> wash cycle without detergent in a dishwasher per ABI instruction. We are
> still getting some sort of buildup that causes very short reads (250-300
> bases) and sometimes causes sequence to stop around 150 bases, start again
> and then stop again. ABI tech service has recommended we try the 2M NaOH
> strip method but I have heard that it may not be the best way to clean the
> plates. I am also not completely convinced that the problem is entirely
> caused by dirty plates. If anyone has any suggestions, I would love to
> hear them. Thanks!
>
> Emily Machelski
>
>
>
>
>
>
> DNA Sequencing Facility
> Protein and Nucleic Acid Chemistry Laboratory
> Washington University School of Medicine
> 660 S. Euclid Ave, Box 8103
> St. Louis, MO 63110
>
> ejm@pharmdec.wustl.edu
> (314) 362-0283
>
>
>
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Leo Kei Iwai - Chemist
Dept of Biophysics
Escola Paulista de Medicina - EPM - UNIFESP
Lab of Transplant Immunology
Instituto do Coracao - Incor - FMUSP
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