We have sequenced peptides which have had DNA covalently attached. No
interference from the presence of the DNA was seen either in the chemistry
or the chromatograms, except at the site of attachment where the amino acid
residue signal was suppressed. Using radioactive DNA, we found peptide-DNA
conjugates to bind to ProSpin at >95% efficiency so I guess ProSorb would
be fine.
Len
>A client has requested that I N-terminally sequence a protien covalently
>bound to DNA. The protein is 120 kDA and the DNA 9 Kb... is this possible?
>What would the chromatograms look like? Use glass fibre filter or Prosorb?
>
>Thanks in advance for any help on this!
*********************************************************************
Dr Len C. Packman
Assistant Director of Research
Protein and Nucleic Acid Chemistry Facility
Department of Biochemistry
University of Cambridge
80 Tennis Court Road
Old Addenbrookes Site
Cambridge, CB2 1GA, UK
Tel: +44 (1223) 333639 (including answerphone)
FAX: +44 (1223) 766002
e-mail: lcp2@mole.bio.cam.ac.uk
Visit my WWW page at http://www.bio.cam.ac.uk/proj/adr/PNAC/pnac.html