extinction coefficient
JohnNakamura@inhale.com
Thu, 9 Jul 1998 17:48:34 -0700
A well characterized protein with known molecular weight has a defined
extinction coefficient for quantitating protein amount by UV analysis.
However, when the protein is lyophilized, the gravimetric weight of the
lyophilized material is much higher by about 20% than the value calculated
from UV analysis. This preparation is also extremely pure which excludes
impurities contributing to the overall weight. What can be happening and
how would you test your hypothesis?