We tried Arg-C and were less than impressed. Lowell Ericson
suggested a classic approach:
Date: Tue, 23 Apr 1996 10:07:07 -0700 (PDT)
From: ERICSSONLH@u.washington.edu
Subject: Substitute for Arg-C
I have heard several complaints about the quality of Arg-C digests.
Consider instead citrconylation of the epsilon amino lysine groups
followed by tryptic cleavage at Arg only. The citroconyl group
can eventually be easily removed by dropping the pH. Check
YE OLD ref. Biochemistry vol 13 3146 (1973).
-Lowell Ericsson
P.S. Citroconylation also helps to solubilize the protein.
David