I second David Schooley's comment about Arg-C being less than impressive
although, in our experience, this poor performance applies to in-gel
digests, for a few cases it has worked satisfactorily in solution and not
at all in-gel.
Lowell's citraconylation method would seem to be a good option but I must
mention that I have , in the distant past, tried this approach in-gel and
had trouble achieving complete blocking of the lysines, there were still
some digestion products of lysine cleavage. I was not in a position, at the
time, to systematically work out where the problem was and I would like to
know if anyone has worked out a good in-gel citraconylation method.
Regards...Ken
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Ken I. Mitchelhill
The John Holt Protein Structure Laboratory
St. Vincent's Institute of Medical Research
41 Victoria Parade
Fitzroy 3065 Victoria
AUSTRALIA
Telephone: 61-3-9288 2480
Facsimile: 61-3-9416 2676
Email: k.mitchelhill@medicine.unimelb.edu.au
Laboratory: http://www.medstv.unimelb.edu.au/WWWDOCS/SVIMRdocs/JHPSL.html
ABRF: http://www.abrf.org
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