RE: CNBr digestion

Brett Phinney (phinney@bchserver.bch.ncsu.edu)
Thu, 16 Jul 1998 13:07:22 -0400

I remember reading somewhere that you can do a in gel CNBr cleavage by
exposing the gel piece to CNBr vapors in a sealed environment. I cannot find
the cite right now but I'll continue to look for it.

good Luck

__________________________________
Brett Phinney
North Carolina State University
Department of Biochemistry
Ph# 919-515-5786, Fax 919-515-2047
ICQ# = 12442943

> -----Original Message-----
> From: Association of Biomolecular Resource Facilities
> [mailto:abrf-request@aecom.yu.edu]On Behalf Of Helene Hill
> Sent: Wednesday, July 15, 1998 5:26 PM
> To: Recipients of ABRF List
> Cc: Helene Hill
> Subject: CNBr digestion
>
>
>
> Hello out there
>
> Can anyone please tell me: can CNBr be used for in-gel digestion? If yes,
> does anyone have a protocol for doing that?
>
> Thank you.
>
> Helene Z Hill, Ph.D. Internet: hill@umdnj.edu
> Head, Section of Cancer Biology Voice/fax: (973)972-3421
> Mail: MSB-E586
> NJ Medical School
> 185 South Orange Ave
> Newark, NJ 07103-2714
>