Re: proteolysis at arg

xiaohui chen (xhchen@biocserver.BIOC.CWRU.Edu)
Thu, 16 Jul 1998 16:47:33 -0400 (EDT)

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At 09:43 AM 7/15/98 -0600, you wrote:
>I have need to cleave a large protein at R in order to identify a
>particular carboxymethy cys residue. Greg Grant suggested that I poll the
>group for advice as to a suitable protease and protocol to do such if there
>is one available. I have about 500 pmol of tritiated protein that is mr
>140000. Thanks for your help Phil Majerus
>
>Phil Majerus
>
>
>

Phil,
If you don't care about modification on Lys, you may try reductive
methylation to convert -NH2 to -N(CH3)2, then desalting followed by tryptic
digestion. The reductive methylation can block Lys completely along with
N-terminal.
Good luck.

Xiaohui

Xiaohui Chen, Ph.D.
Department of Biochemistry
School of Medicine
Case Western Reserve University
2109 Adelbert Road
Cleveland, OH 44106
Ph: 216-368-3250
Fax: 216-368-3419
Email: xhchen@biochemistry.cwru.edu

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