How to get rid of TFA?

Federico Brianza (brianza@imb-jena.de)
Fri, 17 Jul 1998 13:38:58 +0100

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Hi everybody,
after purification with RPC-FPLC, using acetonitril and TFA, my synthetic peptides
have extremely low pH, I guess due to the presence of TFA. The problem persists
also after freeze-drying. What can I do? Is it true that freeze-dryng after adding
acetic acid can help remove the TFA?
Thanks in advance

-- 
Federico Brianza
Protein Laboratory
Institute of Molecular Biotechnology
Beutembergstr. 11, 07745 Jena / Germany
Tel: +49 3641 656432
Fax: + 49 3641 656431

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