In my experience with Arg cleavage (several years ago), I found that
Clostripain worked significantly better than the other endoproteinase
Arg-C enzymes.
Jeff Hulmes
Senior Scientist
Protein/Synthetic Chemistry
ImClone Systems Inc.
180 Varick Street
New York, NY 10014
(212) 645-1405
Fax: (212)645-2054
JeffH@ImClone.com
>>> "phil@im.wustl.edu" 07/15/98 01:15pm >>>
I have need to cleave a large protein at R in order to identify a
particular carboxymethy cys residue. Greg Grant suggested that I poll
the
group for advice as to a suitable protease and protocol to do such if
there
is one available. I have about 500 pmol of tritiated protein that is mr
140000. Thanks for your help Phil Majerus
Phil Majerus