FW: How to get rid of TFA?
Breslav, Michael [PRI] (MBreslav@prius.jnj.com)
Fri, 17 Jul 1998 13:05:30 -0400
> Federico,
> I tried to freeze-dry TFA salts from acetic acid several times measuring
> the amount of TFA before and after and this did not work well. But an
> ion-exchange resin in an acetate form worked fine for my peptides.
> Trifluoroacetate was transferred to an acetate.
> Regards,
> Michael Breslav
> R.W.Johnson PRI
> Spring House, PA
>
> ----------
> From: Federico Brianza[SMTP:brianza@imb-jena.de]
> Sent: Friday, July 17, 1998 8:38 AM
> To: Recipients of ABRF List
> Subject: How to get rid of TFA?
>
> Hi everybody,
> after purification with RPC-FPLC, using acetonitril and TFA, my synthetic
> peptides
> have extremely low pH, I guess due to the presence of TFA. The problem
> persists
> also after freeze-drying. What can I do? Is it true that freeze-dryng
> after adding
> acetic acid can help remove the TFA?
> Thanks in advance
>
>
>
> --
> Federico Brianza
> Protein Laboratory
> Institute of Molecular Biotechnology
> Beutembergstr. 11, 07745 Jena / Germany
> Tel: +49 3641 656432
> Fax: + 49 3641 656431
>
>