TFA can be quite a nuisance after chromatography. Hence there is interest
in finding solutions that either get rid of the TFA salts or avoid them
from the beginning. Let me explain.
1: Clean-up
Michael Breslav suggested ion-exchange resins which appears to me as good
solution. I know also that Axel Ducret did some work in this direction --
perhaps he might eleborate.
in that sense I would also like to add to Jeff Hulmes' remark:
"...remember that RP-HPLC
doesn't work for everything..." that is why there are many other liquid
chromatographic separation techniques....
2 Avoiding TFA in RP-HPLC
To avoid TFA alltogether there is an option in RP-HPLC. The company I'm
with developed a special reverse phase C-18 material that provides good
separations without TFA.
The material is called PepMap. If you'd like more information (technical
or commercial), please contact me by e-mail. I just don't want to
reprimanded for being too commercial after a long absence from this
bulletin board. (And I'm really glad to be back...)
Sincerely: Jean-Pierre Salzmann
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Jean-Pierre Salzmann
LC Packings (USA) Inc.
80 Carolina Street
San Francisco CA 94103
Phone (415) 552-1855
Fax (415) 552-1859
E-mail jpsalzmann@earthlink.net
URL http://www.lcpackings.com
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