I would like to know what other peptide synthesis facility would do if
they were in my situation: a researcher of our university wanted that I
synthesize a peptide (23 mer) that I will call xyz, and wanted some
modifications:
xyz
xyz-CYS
biotin-xyz
biotin -xyz-CYS
(so same peptide but with amino or C-terminus modified= 4 different products)
The C-terminus of the peptide xyz is arginine. He wanted the products 95%
pure and of course wanted the best price.
Would you synthesize it in one step using two resins (preloaded Cys Wang
resin, add Arg and then add Arg preloaded 2 Cltrytil resin) and take
aliquotes at the end for biotinylation? Or would you synthesize it more
than one time or else?
Would you charge for 1,2, 3 or 4 synthesis?
(in fact, I already did the synthesis in one step and charged for one
synthesis + 4 purification but the yield of one of the 4 products is not
enough (5 mg instead of 20) and he wants that I synthesize the peptide
again (free of charge). Before I do anything else for him, I would like to
know if my strategy was right and if my "relative" charge (price) was good
in comparison with other services. I know that the yield would not have
been a problem if I had synthesized them separately.
Thank you very much for your input,
Sylvie
Sylvie Bourassa Ph.D.
Service de Sequence de Peptides de l'Est du Quebec (SSPEQ)
CHUL Research Center Room 9750
2705, Laurier blvd
Ste-Foy, Quebec
Canada G1V 4G2
phone 418 656-4141 ext 7088
fax 418 654-2159
Email: sspeq@crchul.ulaval.ca