Psyn: synthesis strategy and relative cost
Joseph Fernandez (fernaj@rockvax.rockefeller.edu)
Mon, 20 Jul 1998 07:58:32 -0400 (EDT)
>
>To: phil@im.wustl.edu (Philip Majerus)
>From: Joseph Fernandez <fernaj@rockvax.rockefeller.edu>
>Subject: Re: proteolysis at arg
>Cc:
>Bcc:
>X-Attachments:
>
>Phil,
> I have been on vacation for a few weeks and only saw your messgae
>today. All the responses appear to have had problems with Arg-C from
>Boeringer Mannheim which I have also had problems with. One thing not
>mentioned is a different source of an ARG specific protease(Clostipain)
>from Promega. You do have to follow the company's instructures for
>digestion and reconstitution buffers to get adequate results. We have
>used it quite a bit and have observed no trypsin activity. We also got
>many peptides that have one or more internal lysines, confirming we got
>little if any trypsin activity. Best of luck.
>
>Joe
>
>>I have need to cleave a large protein at R in order to identify a
>>particular carboxymethy cys residue. Greg Grant suggested that I poll the
>>group for advice as to a suitable protease and protocol to do such if there
>>is one available. I have about 500 pmol of tritiated protein that is mr
>>140000. Thanks for your help Phil Majerus
>>
>>Phil Majerus
>
>
>
Joseph Fernandez
Associate Director
The Rockefeller university
Protein/DNA Technology Center
1230 York Ave. New York, NY 10021
Phone: (212)-327-8869
FAX : (212)-327-8620
email: fernaj@rockvax.rockefeller.edu
Lab Web Page: http:\\pdtc.rockefeller.edu